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KMID : 0378019720150090087
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New Medical Journal
1972 Volume.15 No. 9 p.87 ~ p.95
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Effects of Some Hormones on the Intestinal Goblet Cells of Adrenalectomized Rats
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Abstract
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This experiment was performed in order to study the influence of adrenalectomy on the intestinal goblet cells of rats and the effects of some hormones on the cells of the adrenalectomized rats.
Healthy adult rats weighing about 200g were divided into three groups: normal, adrenalectomized, and hormone-treated. Adrenalectomy was done under ether anaesthesia. The adrenalectomized rats were killed on the 3rd and 7th day after the operation and compared with those of sham operated. The hormone-treated group was further divided into adrenocorticotropic hormone, cortisone, deoxycorticosterone acetate, and epinephrine sub-groups, and each kind of hormone was injected subcutaneously every day from the 8th post-operative day with a dose of 1.25 1U, 25 mg, I mg and 0. 1 mg per kg body weight respectively. The hormone-treated animals were sacrificed on the 3rd and 7th day from the beginning of the injection. All findings of hormone-treated groups were compared with those of saline injected group.
Duodenal and colonic mucosae of experimental rats were fixed in 10% neutral formalin solution, j embedded in paraffin wax, sectioned in 6 p thickness, and stained with hematoxylin-eosin stain for general findings and with periodic acid-Schiff reaction, alcian blue pH 2. 5, alcian blue pH 1. 0 and combined alcian blue-PAS for the mucosubstance of goblet-cells.
Observed results were as follows:
1. Adrenalectomy caused the decrease of mucosubstance in volume, coagulation or dissolution of mucosubstance of goblet cells. Histochemically, sulfated mucosubstance most sensitively responsed.
2. Adrenocorticotrophic hormone did not influence the mucosubstance of intestinal goblet cells in the adrenalectomized rats.
3. Administration of cortisone, deoxycorticosterone acetate or epineprine to the adrenalectomized rats caused an increase in volume of the neutral and acidic mucosubstances of intestinal goblet cells
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